Nuclear exclusion of transcription factor IIIA and the 42s particle transfer RNA-binding protein in Xenopus oocytes: a possible mechanism for gene control?
نویسندگان
چکیده
The intracellular location of 7S and 42S RNP particles in Xenopus oocytes has been determined by immunohistochemistry. Using antibodies directed against the 48-mol-wt protein component of the 42S particle and against transcription factor IIIA, the protein moiety of the 7S particle, we show that these ribonucleoprotein particles are detectable only in the oocyte cytoplasm, being excluded from the nucleus. The mechanism of this nuclear exclusion, and its possible significance in the regulation of 5S RNA gene expression, are discussed.
منابع مشابه
An alternative protein factor which binds the internal promoter of Xenopus 5S ribosomal RNA genes.
In small oocytes of Xenopus species, two sets of 5S RNA genes, oocyte-type and somatic-type, are fully activated. The 5S RNA transcripts are temporarily stored, half in association with TFIIIA to form a 7S particle, the other half in association with tRNA and two proteins (p48 and p43) to form a 42S particle. It has been established previously that TFIIIA binds to the internal control region of...
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عنوان ژورنال:
- The Journal of Cell Biology
دوره 97 شماره
صفحات -
تاریخ انتشار 1983